Journal
BIOSENSORS & BIOELECTRONICS
Volume 38, Issue 1, Pages 79-85Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2012.05.007
Keywords
Surface-initiated atom radical polymerization; Glycidyl methacrylate; Ferrocenylmethyl methacrylate; Immunosensing; Tumor necrosis factor-alpha
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Funding
- National Basic Research Program of China [2010CB732400]
- Key Program from the National Natural Science Foundation of China [21035002]
- National Natural Science Foundation of China [20875013]
- Key Program from the Natural Science Foundation of Jiangsu province [BK2010059]
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A novel label-free immunosensing strategy for sensitive detection of tumor necrosis factor-alpha antigen (TNF-alpha) via surface-initiated atom transfer radical polymerization (SI-ATRP) was proposed. In this strategy, the Au electrode was first modified by consecutive SI-ATRP of ferrocenylmethyl methacrylate (FMMA) and glycidyl methacrylate (GMA), and TNF-alpha antibody was coupled to the copolymer segment of GMA (PGMA) by aqueous carbodiimide coupling reaction. Subsequently, the target TNF-alpha antigen was captured onto the Au electrode surface through immunoreaction. The whole process was confirmed by scanning electron microscopy (SEM) and surface plasmon resonance (SPR) measurements. With introduction of redox polymer segment of FMMA (PFMMA) as electron-transfer mediator, the antigen-coupled Au electrode exhibited well electrochemical behavior, as revealed by cyclic voltammetry measurement. This provided a sensing platform for sensitive detection of INF-alpha with a low detection limit of 3.9 pg mL(-1). Furthermore, the living characteristics of the ATRP process can not only be readily controlled but also allow further surface functionalization of the electrodes, thus the proposed method presented a way for label-free and flexible detection of biomolecules. (c) 2012 Elsevier B.V. All rights reserved.
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