Journal
BIOSENSORS & BIOELECTRONICS
Volume 36, Issue 1, Pages 56-61Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2012.03.037
Keywords
tmRNA; Silicon photonics; Microring resonator; Bacterial detection; Label-free biosensing; Biomarker
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Funding
- National Institutes of Health (NIH), NIH Roadmap for Medical Research [1-DP2-OD002190-01]
- Center for Advanced Study at the University of Illinois at Urbana-Champaign
- Camille and Henry Dreyfus Foundation
- Estonian Ministry of Education and Research [SF0180026s09, SF0180027s10]
- European Social Fund's Doctoral Studies and Internationalization Program DoRa
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A label-free biosensing method for the sensitive detection and identification of bacterial transfer-messenger RNA (tmRNA) is presented employing arrays of silicon photonic microring resonators. Species specific tmRNA molecules are targeted by complementary DNA capture probes that are covalently attached to the sensor surface. Specific hybridization is monitored in near real-time by observing the resonance wavelength shift of each individual microring. The sensitivity of the biosensing platform allowed for detection down to 53 fmol of Streptococcus pneumoniae tmRNA, equivalent to approximately 3.16 x 10(7) CFU of bacteria. The simplicity and scalability of this biosensing approach makes it a promising tool for the rapid identification of different bacteria via tmRNA profiling. (C) 2012 Elsevier B.V. All rights reserved.
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