Journal
BIOSENSORS & BIOELECTRONICS
Volume 32, Issue 1, Pages 297-299Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.12.007
Keywords
Fluorescence; Nanoclusters; Biosensors; Gold; Protease
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Funding
- National Natural Science Foundation of China [20875086]
- Chinese Academy of Sciences (CAS)
- Academy of Sciences for the Developing World (TWAS)
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In this study, fluorescent metal nanoclusters are presented as novel probes for sensitive detection of protease for the first time. The sensing mechanism is based on trypsin digestion of the protein template of BSA-stabilized Au nanoclusters. The decrease in fluorescence intensity of BSA-Au nanoclusters caused by trypsin allows the sensitive detection of trypsin in the range of 0.01-100 mu g/mL. The detection limit for trypsin is 2 ng/mL (86 pM) at a signal-to-noise ratio of 3. The present nanosensor for trypsin detection possesses red emission, excellent biocompatibility, high selectivity, and good stability. In addition, we demonstrated the application of the present approach in real urine samples, which suggested its potential for diagnostic purposes. (C) 2011 Elsevier B.V. All rights reserved.
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