Journal
BIOSENSORS & BIOELECTRONICS
Volume 38, Issue 1, Pages 19-26Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2012.04.026
Keywords
Localized surface plasmon resonance; Optical biosensor; Arsenic; Binding kinetics; Nanoparticle; 3-D polymer
Categories
Funding
- FIU Graduate School Doctoral Evidence Acquisition Fellowship
- AMERI (Advanced Material Engineering Research Institute, FIU)
- DOD TATRC [W81XWH-10-1-0732]
- U.S. Public Health Service Grant [GM55425]
- NIH [R15 ES021079-01]
- Everglades Foundation
- Kauffman Foundations
Ask authors/readers for more resources
A self-assembled 3D hydrogel-nanoparticle composite integrated surface plasmon resonance (SPR) sensor is reported here. The novel assembled substrate was developed by means of a surface mediated radical co-polymerization process to obtain a highly sensitive hydrogel-based thin film that possesses specific binding sites for target analytes. Initially, amino group modified gold nanoparticles (AuNPs) were covalently linked to acrylic acid monomer. Following this, N-isopropylacrylamide (NIPAAm) and AuNPs linked acrylic acid (AAc) monomers were randomly co-polymerized by the grafting from method in the presence of initiator and crosslinker onto the sensing surface. Surface charecterization techniques were utilized to evaluate the thickness and composition of the hydrogel-nanoparticle film. The sensing platform was employed to study the binding kinetics and conformational changes of the ArsA ATPase as a consequence of binding trivalent arsenicals under a variety of conditions. ArsA, the catalytic subunit of the ArsAB arsenite (As(III)) translocating ATPase, is one of the five proteins encoded by the arsenical resistance (ars) operon of plasmid R773 in cells of Escherichia coli, that confers resistance to trivalent and pentavalent salts of the metalloid arsenic. SPR measurements indicate that the 3D hydrogel-nanoparticle coated sensors exhibited a higher sensitivity than that of the 2D AuNPs decorated sensors. Binding of As(III) to ArsA is greatly facilitated by the presence of magnesium ion and ATP. (c) 2012 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available