Journal
BIOSENSORS & BIOELECTRONICS
Volume 32, Issue 1, Pages 62-68Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.11.023
Keywords
Basophils; Biosensor; Surface plasmon resonance imaging; Single cell analysis; Diagnosis of allergy; Histamine release
Categories
Funding
- Hiroshima Prefectural Institute of Industrial Science and Technology, Japan
- Takeda Science Foundation
- Program for Promotion of Basic and Applied Researches for Innovation in Bio-oriented Industry
- Grants-in-Aid for Scientific Research [21591432] Funding Source: KAKEN
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Basophil activation in response to antigen may represent specificities of type I allergy of individuals and their reactions in the body. We previously demonstrated that surface plasmon resonance (SPR) sensor could detect the activation of human basophils in response to antigens. In this study, we further developed a technique based on SPR imaging (SPRI) system to detect reactions of individual basophils isolated from human blood, and investigated the potential of this sensor as a tool for diagnosis of type I allergy. To detect the change of refractive index (RI) in individual basophils, human basophils were isolated by negative selection with antibodies conjugated with magnetic beads, fixed on a gold film with anti-basophil antibody and stimulated with various antigens under the measurement of SPRI. The sensor could detect the reactions of individual basophils in response to specific antigens as well as non-specific activators. Moreover, the sensor well allocated two spots of basophils on a sensor chip and detected individual reactions to antigen. Thus, the technique developed in this study can visualize the effect of various stimuli or inhibitors on basophils as change of intracellular RI distribution at the single cell level. In combination with a device to rapidly isolate basophils from peripheral blood, this technique may be a useful tool as a high throughput screening system in clinical diagnosis for type I allergy. (C) 2011 Elsevier B.V. All rights reserved.
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