Journal
BIOSENSORS & BIOELECTRONICS
Volume 31, Issue 1, Pages 101-104Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.09.045
Keywords
Horseradish peroxidase; Hydrophilic ionic liquid; Titanate nanotubes; Direct electrochemistry; Gelation
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Funding
- Henan Science and Technology Department [084300510023, 092102310282]
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The direct electrochemistry of horseradish peroxidase (HRP) on a novel sensing platform modified glassy carbon electrode (GCE) has been achieved. This sensing platform consists of Nation, hydrophilic room-temperature ionic liquid (RTIL) and Au nanoparticles dotted titanate nanotubes (GNPs-TNTs). The composite of RTIL and GNPs-TNTs was immobilized on the electrode surface through the gelation of a small amount of HRP aqueous solution. The composite was characterized by transmission electron microscopy (TEM), powder X-ray diffraction (XRD) and infrared spectroscopy (IR). UV-Vis and IR spectroscopy demonstrated that HRP in the composite could retain its native secondary structure and biochemical activity. The HRP-immobilized electrode was investigated by cyclic voltammetry and chronoamperometry. The results from both techniques showed that the direct electron transfer between the nanocomposite modified electrodes and heme in HRP could be realized. The biosensor responded to H2O2 in the linear range from 5 x 10(-6) to 1 x 10(-3) mol L-1 with a detection limit of 2.1 x 10(-6) mol L-1 (based on the S/N = 3). (C) 2011 Elsevier B.V. All rights reserved.
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