Journal
BIOSENSORS & BIOELECTRONICS
Volume 32, Issue 1, Pages 127-132Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.11.045
Keywords
Gold nanoparticles; BRCA-1; Single-mismatched detection; Salt-induced; DNA hybridization
Categories
Funding
- Korea Science and Engineering Foundation (KOSEF)
- National Research Laboratory (NRL) [R0A-2008-000-20078-0]
- Basic Science Research Program [2010-0027771]
- Carbon Dioxide Reduction & Sequestration Research Center [DG2-201]
- Ministry of Science and Technology of the Republic of Korea
- National Research Foundation of Korea [R0A-2008-000-20078-0] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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In this study, a gold nanoparticle (Au-NP)-based detection method for sensitive and specific DNA-based diagnostic applications is described. A sandwich format consisting of Au-NPs/DNA/PMP (Streptavidin-coated MagnetSphere Para-Magnetic Particles) was fabricated. PMPs captured and separated target DNA while Au-NPs modified with oligonucleotide detection sequences played a role in recognition and signal production. Due to the much lower stability of mismatched DNA strands caused by unstable duplex structures in solutions of relatively low salt concentration, hybridization efficiency in the presence of different buffers was well investigated, and thus, the optimized salt concentration allowed for discrimination of single-mismatched DNA (MMT) from perfectly matched DNA (PMT). Therefore, quantitative information concerning the target analyte was translated into a colorimetric signal, which could easily and quantitatively measured by low-cost UV-vis spectrophotometric analysis. The results indicated this to be a very simple and economic strategy for detection of single-mismatched DNA strands. (C) 2011 Elsevier B.V. All rights reserved.
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