Journal
BIOSENSORS & BIOELECTRONICS
Volume 26, Issue 5, Pages 1902-1907Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2010.03.029
Keywords
Biosensors; Gold nanoshells; Localized surface plasmon resonance; L-DOPA; Tyrosinase activity
Categories
Funding
- National Natural Science Foundation of China [90923010]
- National 863 High-Tech Program of China [2007AA022007]
- Specialized Research Fund for the Doctoral Program of Higher Education of China [20090092110026]
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In the present work, we have developed a novel nanocomposite-based method for the detection of L-DOPA and tyrosinase (TR) activity. This was accomplished by growth-sensitive gold nanoshells (GNSs) precursor nanocomposites (SiO2/GNPs II) and the formation process of GNSs. L-DOPA can reduce AuCl4- to Au-0, depositing on the surface of SiO2/GNPs II and mediating the enlargement of gold nanoparticles (GNPs). Here, the preadsorbed GNPs on SiO2/GNPs II serve as nucleation sites for Au-0 deposition. As the concentration of L-DOPA increases, the surface coverage of resultant gold on silica cores increases accordingly until continuous GNSs are formed. In this growth procedure, the spectra changes in wavelength correlate well with the concentration of L-DOPA, which indicates that this nanocomposite is a good nanoprobe for detecting L-DOPA. Because TR can catalyze the hydroxylation of L-tyrosine to form L-DOPA, this approach can also be employed to analyze the activity of TR, which possesses vast clinical and food industrial importance. Crown Copyright (C) 2010 Published by Elsevier B.V. All rights reserved.
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