Journal
BIOSENSORS & BIOELECTRONICS
Volume 26, Issue 6, Pages 3059-3062Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2010.11.040
Keywords
Aptamer; Gold nanoparticle; Strip; Ochratoxin A
Categories
Funding
- National Natural Science Foundation of China [21071066, 20911120035, 20835006]
- 11th Five Years Key Programs for Science and Technology Development of China [2008BAK41B03, 2008ZX08012-001]
- Natural Science Foundation of Jiangsu Province
- MOF
- MOE [BK2010001, BK2010141, 2010DFB3047, JUSRP11019, 201110060, 201110016, 201110061, 201010078, 201010216, 200910013, 200910083, 200910011, 200910277, 200810099, 200810219]
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An aptamer-based chromatographic strip assay method for rapid toxin detection was developed. The aptamer-based strip assay was based on the competition for the aptamer between ochratoxin A and DNA probes. The sensing results indicated that the sensitivity of the aptamer-based strip was better than that of conventional antibody-based strips. The visual limit of detection of the strip for qualitative detection was 1 ng/mL while the LOD for semi-quantitative detection could down to 0.18 ng/mL by using scanning reader. The recoveries of test samples were from 96% to 110%. All detections could be achieved in less than 10 min, indicating that the aptamer-based strip could be a potential useful tool for rapid on-site detections. (C) 2010 Elsevier B.V. All rights reserved.
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