Journal
BIOSENSORS & BIOELECTRONICS
Volume 27, Issue 1, Pages 77-81Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.06.019
Keywords
Darkfield microscopy; Gold nanoparticles; Optical detection; DNA sensing; Local surface plasmon resonance; Biosensors
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Funding
- MicroNed [WP2F-FoaC]
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We introduce a sensing platform for specific detection of DNA based on the formation of gold nanoparticles dimers on a surface. The specific coupling of a second gold nanoparticle to a surface bound nanoparticle by DNA hybridization results in a red shift of the nanoparticle plasmon peak. This shift can be detected as a color change in the darkfield image of the gold nanoparticles. Parallel detection of hundreds of gold nanoparticles with a calibrated true color camera enabled us to detect specific binding of target DNA. This enables a limit of detection below 1.0 x 10(-14) M without the need for a spectrometer or a scanning stage. (C) 2011 Elsevier B.V All rights reserved.
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