A novel chemiluminescence paper microfluidic biosensor based on enzymatic reaction for uric acid determination

In this work, chemiluminescence (CL) method was combined with microfluidic paper-based analytical device (mu PAD) to establish a novel CL mu PAD biosensor for the first time. This novel CL mu PAD biosensor was based on enzyme reaction which produced H2O2 while decomposing the substrate and the CL reaction between rhodanine derivative and generated H2O2 in acid medium. Microchannels in mu PAD were fabricated by cutting method. And the possible CL assay principle of this CL mu PAD biosensor was explained. Rhodanine derivative system was used to reach the purpose of high sensitivity and well-defined signal for this CL mu PAD biosensor. And the optimum reaction conditions were investigated. The quantitative determination of uric acid could be achieved by this CL mu PAD biosensor with accurate and satisfactory result. And this biosensor could provide good reproducible results upon storage at 4 C for at least 10 weeks. The successful integration of mu PAD and CL reaction made the final biosensor inexpensive, easy-to-use, low-volume, and portable for uric acid determination, which also greatly reduces the cost and increases the efficiency required for an analysis. We believe this simple, practical CL mu PAD biosensor will be of interest for use in areas such as disease diagnosis. (C) 2011 Elsevier B.V. All rights reserved.