Journal
BIOSENSORS & BIOELECTRONICS
Volume 29, Issue 1, Pages 184-188Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.08.015
Keywords
Layer-by-layer self-assemblies; Pathogen detection; Quantum dots; Signal amplification; Target recycling
Categories
Funding
- National Natural Science Foundation of China [20905062, 20675064]
- Natural Science Foundation Project of Chongqing City [CSTC-2009BB5052]
- Scientific Research Foundation for the Returned Overseas Chinese Scholars
- China Postdoctoral Science Foundation [20090460715, 201003305]
- Fundamental Research Funds for the Central Universities [XDJK2009B013]
- Chongqing University of Technology
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We report an ultrasensitive electrochemical approach for the detection of uropathogen sequence-specific DNA target. The sensing strategy involves a dual signal amplification process, which combines the signal enhancement by the enzymatic target recycling technique with the sensitivity improvement by the quantum dot (QD) layer-by-layer (LBL) assembled labels. The enzyme-based catalytic target DNA recycling process results in the use of each target DNA sequence for multiple times and leads to direct amplification of the analytical signal. Moreover, the LBL assembled QD labels can further enhance the sensitivity of the sensing system. The coupling of these two effective signal amplification strategies thus leads to low femtomolar (5 fM) detection of the target DNA sequences. The proposed strategy also shows excellent discrimination between the target DNA and the single-base mismatch sequences. The advantageous intrinsic sequence-independent property of exonuclease Ill over other sequence-dependent enzymes makes our new dual signal amplification system a general sensing platform for monitoring ultralow level of various types of target DNA sequences. (C) 2011 Elsevier B.V. All rights reserved.
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