Journal
BIOSENSORS & BIOELECTRONICS
Volume 26, Issue 12, Pages 4774-4778Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.05.048
Keywords
Biosensor; Label-free; Photonic crystal fiber; Long-period grating; Optofluidics
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Funding
- US National Science Foundation [ECCS-0922175]
- Directorate For Engineering
- Div Of Electrical, Commun & Cyber Sys [0922175] Funding Source: National Science Foundation
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Using long-period gratings (LPG) inscribed in photonic crystal fiber (PCF) and coupling this structure with an optically aligned flow cell, we have developed an optofluidic refractive index transduction platform for label-free biosensing. The LPG-PCF scheme possesses extremely high sensitivity to the change in refractive index induced by localized binding event in different solution media. A model immunoassay experiment was carried out inside the air channels of PCF by a series of surface modification steps in sequence that include adsorption of poly(allylamine hydrochloride) monolayer, immobilization of anti-rat bone sialoprotein monoclonal primary antibody, and binding interactions with non-specific goat anti-rabbit IgG (H + L) and specific secondary goat anti-mouse IgG (H + L) antibodies. These adsorption and binding events were monitored in situ using the LPG-PCF by measuring the shift of the core-to-cladding mode coupling resonance wavelength. Steady and significant resonance changes, about 0.75 nm per nanometer-thick adsorbed/bound bio-molecules, have been observed following the sequence of the surface events with monolayer sensitivity, suggesting the promising potential of LPG-PCF for biological sensing and evaluation. (C) 2011 Elsevier B.V. All rights reserved.
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