Journal
BIOSENSORS & BIOELECTRONICS
Volume 28, Issue 1, Pages 112-116Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.07.006
Keywords
Sandwich amperometric immunosensor; Alpha fetoprotein; Prussian blue modified hydroxyapatite; Graphene sheet
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Funding
- Natural Science Foundation of China [21075052, 81000976]
- Natural Science Foundation of Shandong Province [ZR2010BM030, ZR2010BQ010, ZR2010EM063]
- Science and Technology Key Plan Project of Shandong Province [2010GSF10628]
- Development Environmental Protection Industry of Shandong Province
- Science and Technology Development Plan Project of jinan City [201004015]
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A sandwich electrochemical immunosensor for the sensitive determination of alpha fetoprotein (AFP) has been fabricated. Prussian blue modified hydroxyapatite (PB@HAP) was firstly prepared and used as electrochemical label due to the wonderful conductivity and good biocompatibility of HAP. The results proved that the immunosensor fabricated using the label based on PB@HAP loaded with horse radish peroxidase (HRP) and secondary anti-AFP antibody (Ab(2)) (PB@HAP-HRP-Ab(2)) had high sensitivity, and the sensitivity of the label PB@HAP-HRP-Ab(2) was much higher than labels of PB@HAP-Ab(2), PB-HRP-Ab(2) and HAP-HRP-Ab(2). The mixture of graphene sheet (GS) and thionine (TH) was not only used to immobilize anti-AFP antibody (Ab(1)) but also took part in the signal amplification. The amperometric signal increased linearly with AFP concentration in the range of 0.02-8 ng/mL with a low detection limit of 9 pg/mL The immunosensor had the advantages of high sensitivity, good selectivity and good stability, and was applied to the analysis of AFP in serum sample with satisfactory results. Due to the low-cost and easy synthesis of PB@HAP, the screen-printed electrodes could be used instead of the bare glass carbon electrode in order to achieve mass production. In addition, it had potential application in the detection of other tumor markers. (C) 2011 Elsevier B.V. All rights reserved.
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