Journal
BIOSENSORS & BIOELECTRONICS
Volume 26, Issue 8, Pages 3674-3678Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.01.030
Keywords
Photoelectrochemistry; Cytosensor; Aptamer; CdSe semiconductor nanoparticles
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Funding
- National Natural Science Foundation [20905040]
- Program for New Century Excellent Talents in Universities [NCET-08-0878]
- Scientific and Technical Development Project of Qingdao [09-1-3-24-jch]
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A label-free photoelectrochemical cytosensor for highly sensitive and specific detection of Ramos cell was developed based on photoactive films. The films were fabricated by a layer-by-layer (LBL) assembly of positively charged poly(dimethyldiallylammonium chloride) (PDDA) and negatively charged CdSe semiconductor nanoparticles (NPs) capped with mercaptoacetic acid. The resulting modified electrodes were tested as sensors for Ramos cell through the recognition of DNA aptamer which was covalently bound to the electrode using the classic coupling reactions between -COOH groups on the surfaces of CdSe NPs and -NH2 groups of the aptamer. The newly developed cytosensor exhibited excellent sensitivity and selectivity. The linear range was from 160 to 1600 cells/mL and the detection limit was 84 cells/mL. (C) 2011 Elsevier B.V. All rights reserved.
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