Journal
BIOSENSORS & BIOELECTRONICS
Volume 30, Issue 1, Pages 235-240Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.09.018
Keywords
Mercury ion; Surface plasmon resonance; Thymine-thymine mismatch; Turn-on detection; Hairpin probe
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Funding
- Department of Health, Executive Yuan [DOH99-TD-N-111-009]
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Inorganic mercury ion (Hg2+) has been shown to coordinate to DNA duplexes that feature thymine-thymine (T-T) base pair mismatches. This observation suggests that an Hg2+-induced conformational change in a single-stranded DNA molecule can be used to detect aqueous Hg2+. Here, we have developed an analytical method using surface plasmon resonance (SPR) to develop a highly selective and sensitive detection technique for Hg2+ that takes advantage of T-Hg2+-T coordination chemistry. The general concept used in this approach is that the turn-on reaction of a hairpin probe via coordination of Hg2+ by the T-T base pair results in a substantial increase in the SPR response, followed by specific hybridization with a gold nanoparticle probe to amplify the sensor performance. Meanwhile, the limit of detection is 1 nM, which is lower than other recently developed techniques. A linear correlation is observed between the measured SPR reflectivity and the logarithm of the Hg2+ concentration over the concentration range of 5-5000 nM. Additionally, the SPR system provides high selectivity for Hg2+ in the presence of other divalent metal ions up to micromolar concentration levels. The proposed approach is also successfully utilized for the determination of Hg2+ in water samples. (C) 2011 Elsevier B.V. All rights reserved.
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