Journal
BIOSENSORS & BIOELECTRONICS
Volume 26, Issue 8, Pages 3714-3718Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.02.024
Keywords
Nanoporous gold; Prostate specific antigen; Immunosensor; Label-free; Cancer biomarker
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Funding
- Natural Science Foundation of China [21075052, 81000976]
- Natural Science Foundation of Shandong Province [ZR2010BM030, ZR2010BQ010]
- Science and Technology Key Plan Project of Shandong Province [2010GSF10628]
- Science and Technology Development Plan Project of Jinan City [201004015]
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Nanoporous gold (NPG) film modified electrode for the construction of novel label-free electrochemical immunosensor for ultrasensitive detection of cancer biomarker prostate specific antigen (PSA) is described. Due to its high conductivity, large surface area, and good biocompatibility, NPG film modified electrode was used for the adsorption of anti-PSA antibody (Ab). The sensing signal is based on the monitoring of the electrode's current response towards K3Fe(CN)(6), which is extremely sensitive to the formation of immunocomplex within the nanoporous film. Under optimum conditions, the amperometric signal decreases linearly with PSA concentration (0.05-26 ng/mL), resulting in a low limit of detection (3 pg/mL). We demonstrated the application of the novel immunosensor for the detection of PSA in real sample with satisfactory results. (C) 2011 Elsevier B.V. All rights reserved.
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