Journal
BIOSENSORS & BIOELECTRONICS
Volume 26, Issue 12, Pages 4697-4701Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.05.017
Keywords
Point mutation; Chemiluminescence; Strand-displacement amplification; DNA biosensor
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Funding
- National Basic Research Program of China [2010CB732404]
- Research Initial Funding Project for Doctors in Qingdao University of Science and Technology [0022335]
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Single nucleotide polymorphism (SNP) genotyping is attracting extensive attentions owing to its direct connections with human diseases including cancers. Here, we have developed a highly sensitive chemiluminescence biosensor based on circular strand-displacement amplification and the separation by magnetic beads reducing the background signal for point mutation detection at room temperature. This method took advantage of both the T4 DNA ligase recognizing single-base mismatch with high selectivity and the strand-displacement reaction of polymerase to perform signal amplification. The detection limit of this method was 1.3 x 10(-16) M, which showed better sensitivity than that of most of those reported detection methods of SNP. Additionally, the magnetic beads as carrier of immobility was not only to reduce the background signal, but also may have potential apply in high through-put screening of SNP detection in human genome. (C) 2011 Elsevier B.V. All rights reserved.
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