Journal
BIOSENSORS & BIOELECTRONICS
Volume 26, Issue 11, Pages 4503-4507Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.05.010
Keywords
Nanopipette; Label-free; Nanopore; Aptamer; Thrombin; STING
Categories
Funding
- National Aeronautics and Space Administration [NNX08BA47A, NNX10AQ16A]
- National Institutes of Health [P01-HG000205]
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Signal Transduction by Ion NanoGating (STING) is a label-free technology based on functionalized quartz nanopipettes. The nanopipette pore can be decorated with a variety of recognition elements and the molecular interaction is transduced via a simple electrochemical system. A STING sensor can be easily and reproducibly fabricated and tailored at the bench starting from inexpensive quartz capillaries. The analytical application of this new biosensing platform, however, was limited due to the difficult correlation between the measured ionic current and the analyte concentration in solution. Here we show that STING sensors functionalized with aptamers allow the quantitative detection of thrombin. The binding of thrombin generates a signal that can be directly correlated to its concentration in the bulk solution. (C) 2011 Elsevier B.V. All rights reserved.
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