Journal
BIOSENSORS & BIOELECTRONICS
Volume 26, Issue 9, Pages 3973-3976Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.03.007
Keywords
Glucose oxidase; Biosensor; Sol-gel; Covalent immobilization; Prussian blue; Multi-walled carbon nanotubes
Categories
Funding
- National Natural Science Foundation of China [NSFC-20977021, 30970829]
- State Key Lab of Urban Water Resource and Environment [2010TS07]
Ask authors/readers for more resources
A novel electrochemical glucose biosensor was developed based on in situ covalent immobilization of glucose oxidase (GOx) by one-pot chitosan (CS)-incorporated sol-gel process in the presence of Prussian blue deposited multi-walled carbon nanotubes hybrids (PB/MWNTs) using 3-isocyanatopropyltriethoxysilane (ICPTES) as both a sol-gel precursor and a covalent coupling agent for GOx and CS. The electrode modified with the PB/MWNTs-GOx-CS-ICPTES sal-gel composite film showed good electrical conductivity and effective low-potential electron transfer mediation toward H(2)O(2) reduction attributed to the incorporation of PB/MWNTs. The biosensor exhibited a linear response to glucose in the concentration range from 2.5 x 10(-5) to 1.3 x 10(-3) M with a correlation coefficient of 0.9998, a detection limit of 7.5 x 10(-6) M, a low response time (10 s), good sensitivity and high anti-interference ability. Compared with the control biosensor based on the traditional tetraethoxysilane derived sal-gel composite film, the biosensor showed a similarly small apparent Michaelis-Menten constant of 3.67 mM but much higher electrochemical and biosensing stability. (C) 2011 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available