Journal
BIOSENSORS & BIOELECTRONICS
Volume 26, Issue 7, Pages 3160-3166Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2010.12.019
Keywords
Gold nanoparticles; Label-free; Thrombin; Fibrinogen; Plasma
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Funding
- National Science Council of Taiwan [99-2113-M-019-001-MY2]
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In this paper, we describe two simple, label-free, homogenous assays using gold nanoparticles (Au NPs) one to detect coagulation-related proteins and the other to screen inhibition reactions. The first nanosensor functions on the basis of the fact that thrombin catalyzes fibrinogen to form long-chain fibrins, which then induce aggregation of Au NPs. We applied this sensor to study the interactions of thrombin, inhibitors, cofactors, and antidotes. We further used thrombin-conjugated Au NPs (Thr-Au NPs) to analyze the levels of fibrinogen in plasma samples via fibrinogen-induced aggregation of Thr-Au NPs. The limit of detection (LOD; S/N =3) of this sensor for fibrinogen in plasma was 10 nM. The Thr-Au NP probe provided quantitative results for fibrinogen in plasma samples that correlated (R-2= 0.97) with those obtained using a clinical von Clauss clotting rate assay. In addition, the Thr-Au NP-based sensor could be used to monitor thrombin concentrations in plasma samples under physiological conditions. Compared with conventional assays, these label-free assays offer several advantages, such as rapid and simple readout by the naked eye or by UV-vis absorption spectroscopy. (C) 2010 Elsevier B.V. All rights reserved.
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