Journal
BIOSENSORS & BIOELECTRONICS
Volume 26, Issue 10, Pages 4177-4183Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.04.022
Keywords
HSA immuonosensor; Poly vinyl alcohol; Conjugate gold nanoparticle; monoclonal; Ab; Differential pulse voltammetry; Square wave voltammetry
Categories
Funding
- Endocrinology and Metabolism Research Center of Tehran University of Medical Sciences, Tehran, I.R. Iran
Ask authors/readers for more resources
The development of immunosensors with high sensitivity and specificity in detecting the pathogenic or physiologically relevant molecules in the body, offers a powerful opportunity in early diagnosis and treatment of diseases. In this study, we developed a new competitive immunosensor with employing antibody (Ab) labeled AuNP (Ab-AuNP) and PVA modified screen-printed carbon electrode (SPCE) surface to detect the urine albumin. Field emission scanning electron microscopy (FE-SEM) of modified electrode showed a suitable and stable attachment between HSA antigen-mAb and AuNP. Cyclic voltammetric (CV) method demonstrated that modification process was well performed. Electrochemical measurements including differential pulse voltammetry (DPV) and square wave voltammetry (SWV) were employed for quantitative antigen detection. The electrochemical measurements performed with other proteins mixed with samples demonstrated a high specificity and selectivity for this biosensor in detecting the USA. In optimal conditions, the immunosensor could detect USA in a high linear range (from 2.5 to 200 mu g/mL) with a low detection limit of 25 ng/ml.. This new strategy could be improved and applied to detect the other antigen. (C) 2011 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available