Journal
BIOSENSORS & BIOELECTRONICS
Volume 26, Issue 6, Pages 2835-2840Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2010.11.008
Keywords
Immunochromatographic electrochemical biosensor; Biomarker; Organophosphorus insecticides; Trichloropyridinol; Competitive immunoreaction
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Funding
- Centers for Disease Control/National Institute for Occupational Safety and Health [R01 OH008173-01]
- National institutes of Health through the National Institute of Neurological Disorders and Stroke [U01 NS058161-01]
- Key Project in the National Science & technology Pillar Program [2009BADB9B03]
- Department of Energy's Office of Biological and Environmental Research located at PNNL
- DOE by Battelle [DE-AC05-76L01830]
- China Scholarship Council
- PNNL
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We present a novel portable immunochromatographic electrochemical biosensor (IEB) for simple, rapid, and sensitive biomonitoring of trichloropyridinol (TCP), a metabolite biomarker of exposure to organophosphorus insecticides. Our new approach takes the advantage of immunochromatographic test strip for a rapid competitive immunoreaction and a disposable screen-printed carbon electrode for a rapid and sensitive electrochemical analysis of captured HRP labeling. Several key experimental parameters (e.g. immunoreaction time, the amount of HRP labeled TCP, concentration of the substrate for electrochemical measurements, and the blocking agents for the nitrocellulose membrane) were optimized to achieve a high sensitivity, selectivity and stability. Under optimal conditions, the IEB has demonstrated a wide linear range (0.1-100 ng/ml) with a detection limit as low as 0.1 ng/ml TCP. Furthermore, the IEB has been successfully applied for biomonitoring of TCP in the rat plasma samples with in vivo exposure to organophosphorus insecticides like Chlorpyrifos-oxon (CPF-oxon). The IEB thus opens up new pathways for designing a simple, rapid, clinically accurate, and quantitative tool for TCP detection, as well as holds a great promise for in-field screening of metabolite biomarkers, e.g., TCP, for humans exposed to organophosphorus insecticides. (C) 2010 Elsevier B.V. All rights reserved.
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