Journal
BIOSENSORS & BIOELECTRONICS
Volume 25, Issue 6, Pages 1434-1438Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2009.10.045
Keywords
Fullerene; Ferrocene; Ionic liquid; Glucose; Biosensor
Categories
Funding
- National Natural Science Foundation of China [20771045, 20676052]
- National High Technique Development Plan of 863 [2007AA10Z428]
- Qing Lan Project
- Natural Science Foundation of Zhejiang Province [Y4080404]
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The paper describes an ingenious approach for the fabrication of a promising glucose sensor, GOx/C-60-Fc-CS-IL, that exploits the synergistic beneficial characteristics of fullerene (C-60), ferrocene (Fc), chitosan (CS) and ionic liquid (IL) for glucose oxidase (GOx). Cyclic voltammetry, impedance spectroscopy and chronoamperometry were used to evaluate performance of the biosensor, respectively. Since efficient electron transfer between GOx and the electrode was achieved, the biosensor exhibits a high sensitivity (234.67 nA nM(-1) cm(-2)), low detection limit (S/N = 3) (3 x 10(-9) M), fast response time (0.752 s), wide calibration range (from 1 x 10(-8) M to 1 x 10(-5) M) and excellent long-term stability (30 weeks). The apparent Michaelis-Menten constant (K-M) of GOx on the composite medium, 0.03 mM, shows high bioelectrocatalytic activity of immobilized enzyme toward glucose oxidation. Due to low operating potential (100 mV), the biosensor is relatively insensitive to electroactive interfering species in human blood such as ascorbic acid, and uric acid. which are commonly found in blood samples. Excellent electrochemical reversibility, high sensitivity and stability, technically simple and possibility of preparation at short period of time are of great advantages of these glucose biosensors. (c) 2009 Elsevier B.V. All rights reserved.
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