Journal
BIOSENSORS & BIOELECTRONICS
Volume 25, Issue 11, Pages 2543-2547Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2010.04.009
Keywords
Telomerase; Cancer cell; Chronocoulometry; Au nanoparticle; Bio-barcode
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Funding
- Education Administration Foundation of Shandong Province [J09LB04]
- Natural Science Foundation of Shandong Province [Y2008B02]
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In the present study, an electrochemical method for highly sensitive detection of human telomerase activity was developed based on bio-barcode amplification assay. Telomerase was extracted from HeLa cells, then the extract was mixed with telomerase substrate (TS) primer to perform extension reaction. The extension product was hybridized with the capture DNA immobilized on the Au electrode and then reacted with the signal DNA on Au nanoparticles to form a sandwich hybridization mode. Electrochemical signals were generated by chronocoulometric interrogation of [Ru(NH(3))(6)](3+) that quantitatively binds to the DNA on Au nanoparticles via electrostatic interaction. This method can detect the telomerase activity from as little as 10 cultured cancer cells without the polymerase chain reaction (PCR) amplification of telomerase extension product. (C) 2010 Elsevier B.V. All rights reserved.
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