Journal
BIOSENSORS & BIOELECTRONICS
Volume 25, Issue 10, Pages 2238-2243Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2010.02.033
Keywords
O-HTCC nanoparticle; Enzyme immobilization; Onion membrane; Glucose oxidase
Categories
Funding
- National Outstanding Youth Research Foundation of China [40925010]
- National Natural Science Foundation of China [40920134003, 40873060, 40673065]
- Chinese Ministry of Science and Technology
- 111 Project [B08030]
- Research Grants Council of the Hong Kong Special Administrative Region, China [HKBU 2006/06P]
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A glucose biosensor comprising a glucose oxidase/O-(2-hydroxyl)propyl-3-trimethylammonium chitosan chloride nanoparticle (O-HTCC NP)-immobilized onion inner membrane and a dissolved oxygen (O-2) sensor has been successfully developed. The detection scheme is based on the depletion of dissolved O-2 content upon exposure to glucose. The decrease in O-2 level was monitored and related to the glucose concentration. The biosensor shows linear response to glucose from 0.0 to 0.60 mM with a detection limit of 50 mu M (S/N = 3). The effect of O-HTCC NP and enzyme loading, pH, temperature, and phosphate buffer concentration on the sensitivity of the biosensor was studied in detail. The biosensor exhibits fast response time (70 s), good repeatability (3.2%, n = 10) and storage stability (90% of initial sensitivity after 3-week storage). Common interferents including acetic acid, lactic acid, propionic acid, butyric acid, folic acid, methanol, glycine, DL-alpha-alanine and DL-cysteine do not cause significant interferences on the biosensor. The proposed biosensor method was successfully applied to determine the glucose content in real samples such as orange juice, red wine and tea drink and the results were comparable to that obtained from a spectrophotometric method. The glucose recovery test demonstrates that the proposed glucose biosensor offers an excellent, accurate and precise method for the determination of glucose in real samples. (C) 2010 Elsevier B.V. All rights reserved.
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