Journal
BIOSENSORS & BIOELECTRONICS
Volume 25, Issue 10, Pages 2296-2301Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2010.03.016
Keywords
Cortisol; Self-assembled monolayer; Electrochemical impedance; Immunosensor; Dithiobis(succinimidyl propionate); Disposable biosensor
Categories
Funding
- NIH [IR43MH085474-01]
- Instacortisol: A Realtime and Continuous Assessment of Cortisol in ISF and USF BITT Award
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Gold microelectrode arrays functionalized with dithiobis(succinimidyl propionate) self-assembled monolayer (SAM) have been used to fabricate an ultrasensitive, disposable, electrochemical cortisol immunosensor. Cortisol specific monoclonal antibody (C-Mab) was covalently immobilized on the surface of gold microelectrode array and the sensors were exposed to solutions with different cortisol concentration. After C-Mab binding, unreacted active groups of DTSP were blocked using ethanol amine (EA) and label-free electrochemical impedance (EIS) technique was used to determine cortisol concentration. EIS results confirmed that EA/C-Mab/DTSP/Au based biosensor can accurately detect cortisol in the range of 1 pM-100 nM. The biosensor was successfully used for the measurement of cortisol in interstitial fluid in vitro. This research establishes the feasibility of using impedance based biosensor architecture for disposable, wearable cortisol detector. (C) 2010 Elsevier B.V. All rights reserved.
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