Journal
BIOSENSORS & BIOELECTRONICS
Volume 25, Issue 9, Pages 2084-2088Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2010.02.004
Keywords
DNA; Electrochemical biosensor; Gold nanoparticle; Diazotization-coupling reaction; 4-Aminothiophenol
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Funding
- National Basic Research Program of China (973 Program) [2007CB936000]
- National Natural Science Funds for Distinguished Young Scholar [20725518]
- National Natural Science Foundation of China [90713019, 20875058, 20775039]
- Science and Technology Development Programs of Shandong Province of China [2008GG30003012]
- Natural Science Foundation of Shandong Province of China [ZR2009BM031]
- Open Foundation of State Key Laboratory of Electroanalytical Chemistry [2009004]
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A novel protocol for the gold nanoparticles (AuNPs) modification on the electrode surface was proposed, which was based on the self-assembly of AuNPs on the mercapto-diazoaminobenzene monolayer modified electrode. The mercapto-diazoaminobenzene monolayer was obtained by covalent immobilization of 4-aminothiophenol (4-ATP) molecules onto another 4-ATP monolayer functionalized gold electrode by diazotization-coupling reaction. The DNA immobilization and hybridization on the AuNPs modified electrode was further investigated. The prepared AuNPs-ATP-diazo-ATP film demonstrated efficient electron transfer ability for the electroactive species toward the electrode surface due to a large conjugated structure of the mercapto-diazoaminobenzene monolayer. The recognition of fabricated electrochemical DNA biosensor toward complementary single-stranded DNA was determined by differential pulse voltammetry with the use of Co(phen)(3)(3+) as an electrochemical indicator. A linear detection range for the complementary target DNA was obtained from 3.01 x 10(-18) to 1.32 x 10(-8) M with a detection limit of 9.10 x 10(-11) M. The fabricated biosensor also possessed good selectivity and could be regenerated easily. (C) 2010 Elsevier B.V. All rights reserved.
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