Journal
BIOSENSORS & BIOELECTRONICS
Volume 25, Issue 5, Pages 1225-1228Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2009.09.025
Keywords
Autodisplay; Z-domain; C-reactive protein; SPR biosensor
Categories
Funding
- National Research Foundation [R15-2004-024-000000-0, 2009-008-1529]
- Ministry of Knowledge Economy
- Seoul Research and Business Development Program [10816]
- Korea Institute of Industrial Technology(KITECH) [10030573] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2009-0073809] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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In this work, the Z-domain of protein A was expressed as a recombinant-fusion protein on the outer membrane of Escherichia coli by using Autodisplay technology, and the outer membrane with Z-domains was layered on a SPR biosensor as a highly sensitive molecular recognition layer. The outer membrane layer formation was demonstrated by using various surfaces with controlled hydrophobicity. The effect of orientation control by the outer membrane with Z-domains was estimated to have far higher sensitivity and far improved limit of detection (LOD) in comparison to the conventional immunoassay by using a model analyte, horseradish peroxidase (HRP). The SPR biosensor with the outer membrane layer was applied to the detection of C-reactive protein (CRP). From these experiments, the LOD of SPR biosensor was estimated to improve more than 100-fold compared to the SPR biosensor with the antibody-layer by physical adsorption. (C) 2009 Elsevier B.V. All rights reserved
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