Journal
BIOSENSORS & BIOELECTRONICS
Volume 25, Issue 5, Pages 1240-1243Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2009.09.040
Keywords
SERS-active substrate; Nanotube array; Optofluidic device; Aptamer; Vasopressin
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Funding
- National Institutes of Health - National Institute of Biomedical Imaging and Bioengineering (NIH-NIBIB) [R21EB007031]
- National Science Foundation [ECS-9731293]
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Here we present an optofluidic surface enhanced Raman spectroscopy (SERS) device for on-chip detection of vasopressin using an aptamer based binding assay. To create the SERS-active substrate, densely packed, 200 nm diameter, metal nanotube arrays were fabricated using an anodized alumina nanoporous membrane as a template for shadow evaporation. We explore the use of both single layer Au structures and multilayer Au/Ag/Au structures and also demonstrate a facile technique for integrating the membranes with all polydimethylsiloxane (PDMS) microfluidic devices. Using the integrated device, we demonstrate a linear response in the main detection peak intensity to solution phase concentration and a limit of detection on the order of 5.2 mu U/mL This low limit of detection is obtained with device containing the multilayer SERS substrate which we show exhibits a stronger Raman enhancement while maintaining biocompatibility and ease or surface reactivity with the capture probe. (C) 2009 Elsevier B.V. All rights reserved.
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