Journal
BIOSENSORS & BIOELECTRONICS
Volume 24, Issue 5, Pages 1399-1404Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2008.08.012
Keywords
Surface plasmon resonance; Biosensors; DNA chip; SPR imaging; Foodborne pathogens
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High-throughput surface plasmon resonance (SPR) biosensor for rapid and parallelized detection of nucleic acids identifying specific bacterial pathogens is reported. The biosensor consists of a high-performance SPR imaging sensor with polarization contrast and internal referencing (refractive index resolution 2 x 10(-7) RIU) and an array of DNA probes microspotted on the surface of the SPR sensor. It is demonstrated that short sequences of nucleic acids (20-23 bases) characteristic for bacterial pathogens such as Brucella abortus, Escherichia coli, and Staphylococcus aureus can be detected at 100 pM levels. Detection of specific DNA or RNA sequences can be performed in less than 15 min by the reported SPR sensor. (C) 2008 Elsevier B.V. All rights reserved.
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