Journal
BIOSENSORS & BIOELECTRONICS
Volume 24, Issue 7, Pages 2277-2280Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2008.11.004
Keywords
Immunochromatographic assay; Colloidal gold particle; Antibody; Indomethacin
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Funding
- National Nature Science Foundation of China (NSFC) [20675054]
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One-step membrane-based competitive immunochromatographic assay using colloidal gold-antibody probe for rapid detection of indomethacin (IDM) in water samples was developed. A positive reaction as a result of the remaining gold-antibody conjugate combining with antigen coated on the membrane was obvious by visual detection. Under optimal conditions, the visual detection limit was found to be 0.1 ng mL(-1), which was about 10-1000 times lower than those in published literatures, demonstrating the high sensitivity of the assay. The stability test indicated the immunochromatographic strips could be used for 8 weeks at room temperature without significant loss of activity. Real water samples were detected by the assay and confirmed by enzyme-linked immunosorbent assay. The assay time for indomethacin detection was less than 10 min, suitable for rapid testing on-site. (C) 2008 Elsevier B.V. All rights reserved.
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