Journal
BIOSENSORS & BIOELECTRONICS
Volume 24, Issue 8, Pages 2696-2701Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2009.01.046
Keywords
Biosensor; Fiber-optic; Evanescent wave; Interleukin; Fluorescence; Cytokine
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We are reporting highly specific and sensitive detection of human interleukin-6 (IL-6) protein using combination tapered fiber-optic biosensor (CTFOB) dip-probe. With these probes we could successfully detect IL-6, down to a concentration of 5 pM (0.12 ng/ml) in the presence of much higher concentration of a non specific protein. Sandwich immunoassay was used to generate specific fluorescence signal. A novel strategy is developed to eliminate the false signal from non specific binding. In this new strategy it is not required to pre-treat probe surface with any kind of blocking buffer. The specificity of the sensor was established by incubating negative control probes in high concentration (1 nM) of another cytokine IL-8. (C) 2009 Elsevier B.V. All rights reserved.
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