Journal
BIOSENSORS & BIOELECTRONICS
Volume 25, Issue 2, Pages 469-474Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2009.08.002
Keywords
Anthrax; Protective antigen; Peptide immobilization; Square wave voltammetry; Biosensor
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Funding
- Hanyang University [HYU-2008-T]
- BioGreen [20080401034006]
- Rural Development Administration, The Republic of Korea
- Rural Development Administration (RDA), Republic of Korea [20080401034006] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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A short chain peptide (26mer) has been successfully utilized for the selective electrochemical detection of the protein biomarker, protective antigen (PA), for the diagnosis of Anthrax. The major motivation of using a peptide instead of an antibody for the development of a biosensor is that there are advantages associated with the smaller size, better biological stability and easy synthesizability of a peptide. PA-selective peptide was synthesized and conjugated on a binding layer previously immobilized onto gold electrode. The same sensing scheme using a conventional antibody instead of a peptide was also tested for the purpose of comparison. Since the size of the peptide is approximately 1-2 orders of magnitude smaller than that of the antibody, a more populated immobilization of peptide on the sensing layer is possible and will eventually lead to improved sensitivity for PA detection. The selectivity of the peptide-based sensor was evaluated by observing the reduction peaks of samples containing PA with different concentrations of bovine serum albumin (BSA). The resulting responses were interference-free from BSA. The results of this study demonstrate the strong analytical potential of a peptide-based biosensor for diverse applications, especially in disease diagnosis where detection of a specific protein biomarker is particularly demanding. (C) 2009 Elsevier B.V. All rights reserved.
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