Journal
BIOSENSORS & BIOELECTRONICS
Volume 25, Issue 2, Pages 373-377Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2009.07.016
Keywords
Nitrogen-doped carbon nanotubes; Glucose oxidase; Direct electrochemistry; Biosensor; Biocompatibility
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Funding
- National Science Funds for Creative Research Groups [20821063]
- Major Research Plan [90713015]
- National Natural Science Foundation of China [20535010, 20705012, 20875044]
- Natural Science Foundation of Jiangsu Province [BK2008014]
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A novel biosensor for glucose was prepared by immobilizing glucose oxidase (GOx) on nitrogen-doped carbon nanotubes (CNx-MWNTs) modified electrode. The CNx-MWNTs membrane showed an excellent electrocatalytic activity toward the reduction of O-2 due to its diatomic side-on adsorption on CNx-MWNTs. The nitrogen doping accelerated the electron transfer from electrode surface to the immobilized GOx, leading to the direct electrochemistry of GOx. The biofunctional surface showed good biocompatibility, excellent electron-conductive network and large surface-to-volume ratio, which were characterized by scanning electron microscopy, contact angle and electrochemical impedance technique. The direct electron transfer of immobilized GOx led to stable amperometric biosensing for glucose with a linear range from 0.02 to 1.02 mM and a detection limit of 0.01 mM (S/N = 3). These results indicated that CNx-MWNTs are good candidate material for construction of the third-generation enzyme biosensors based on the direct electrochemistry of immobilized enzymes. (C) 2009 Elsevier B.V. All rights reserved.
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