Journal
BIOSENSORS & BIOELECTRONICS
Volume 24, Issue 12, Pages 3436-3441Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2009.04.008
Keywords
Surface plasmon resonance; Capacitance; Label-free immunosensor; Cancer antigen 125
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Funding
- Thailand Research Fund
- Center for Innovation in Chemistry (PERCH-CIC)
- Trace Analysis and Biosensor Research Center
- Graduate School and Faculty of Science
- Prince of Songkla University, Hat Yai, Thailand
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This paper presents a comparison between surface plasmon resonance (SPR) and capacitive immunosensors for a flow injection label-free detection of cancer antigen 125 (CA 125) in human serum. Anti-CA 125 was immobilized on gold surface through a self-assembled monolayer. Parameters affecting the responses of each system were optimized. Under optimal conditions, SPR provided a detection limit of 0.1 U ml(-1) while 0.05 U ml(-1) was obtained for the capacitive system. Linearity for SPR was between 0.1 and 40 U ml(-1) and 0.05-40 U ml(-1) for capacitive system. These immunosensors were applied to analyze CA 125 concentrations in human serum samples and compared with conventional enzyme linked fluorescent assay (ELFA). Both systems showed good agreement with ELFA (P < 0.05). Moreover, these immunosensors were very stable and provided good reproducible responses after regeneration, up to 32 times for SPR and 48 times for capacitive system with relative standard deviation lower than 4%. The SPR immunosensor provided advantages in term of fast response and real-time monitoring while capacitive immunosensor offered a sensitive and cost-effective method for CA 125 detection. (C) 2009 Elsevier B.V. All rights reserved.
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