Journal
BIOSENSORS & BIOELECTRONICS
Volume 24, Issue 5, Pages 1184-1188Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2008.07.002
Keywords
Fructose; Fructose dehydrogenase; Electron transfer reaction; Catalytic current; Carbon nanotube
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Multi-walled carbon nanotubes (MWCNTs) were synthesized on platinum plate electrodes by the chemical vapor deposition (CVD) method. From the results of X-ray photoelectron spectroscopy and voltammetric investigation, the iron nanoparticles used as a catalyst for the MWCNT synthesis were enclosed with MWCNTs. The MWCNTs synthesized on the Pt plate (MWCNTs/Pt) electrode were immediately immersed into solutions Of D-fructose dehydrogenase (FDH) to immobilize the enzyme onto the MWCNTs/Pt electrode surfaces. After the FDH was immobilized onto the MWCNTs/Pt electrode, a well-defined catalytic oxidation current based on FDH was observed from ca. -0.15 V (versus Ag/AgCl/sat'd KCl), which was close to the redox potential of heme c as a prosthetic group of FDH. From an analysis of a plot of the catalytic current versus Substrate, the calibration range for the fructose concentration was up to ca. 40 mmol dm(-3), and the apparent Michaelis-Menten constant was evaluated to be 11 +/- 1 mmol dm(-3). (c) 2008 Elsevier B.V. All rights reserved.
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