Journal
BIOSENSORS & BIOELECTRONICS
Volume 24, Issue 7, Pages 2008-2014Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2008.10.004
Keywords
Nanostructured conducting polymer; Polydiphenylamine; Glucose oxidase; Glucose; Amperometric biosensor
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Funding
- Kyungpook National University Research Fund
- Korean Research Foundation [KRF-2006-C00001]
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Nanostructured, hollow spheres of polydiphenylamine (HS-PDPA) are prepared through a soft template assisted self-assembly approach. An enzymatic glucose biosensor is fabricated through immobilizing glucose oxidase (GOx) into HS-PDPA matrix. The HS-PDPA-GOx electrode exhibits a pair of well-defined reversible redox peaks with a fast heterogeneous electron transfer rate. At an applied potential of +0.65 V, HS-PDPA-GOx electrode possesses high sensitivity (1.77 mu A mM(-1) cm(-2)), stability and reproducibility towards glucose. The amperometric current response of HS-PDPA-GOx to glucose is linear in the concentration range between 1 and 28 mM with a detection limit of 0.05 mM (S/N = 3). Also, HS-PDPA-GOx electrode shows high selectivity towards glucose in the presence of ascorbic acid, uric acid and acetaminophen at their maximum physiological concentrations. (C) 2008 Elsevier B.V. All rights reserved.
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