Journal
BIOSENSORS & BIOELECTRONICS
Volume 24, Issue 10, Pages 3049-3054Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2009.03.022
Keywords
Cyanoethyl cellulose; Hemoglobin; Direct electrochemistry; Nitric oxide; Electrocatalysis; Biosensors
Categories
Funding
- Key Project of Chinese Ministry of Education [206104]
- Hunan Provincial Natural Science Foundation of China
- Open Project Program of Key Laboratory of Environmentally Friendly Chemistry
- Applications of Ministry of Education
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A water-soluble cyanoethyl cellulose (CEC), homogeneously synthesized in NaOH/urea aqueous solution, Was used as an immobilization matrix to entrap proteins and enzymes. Then hemoglobin (Hb) was used as a template to fabricate CEC-Hb biomimetic membranes in which the Hb showed direct electrochemistry on a glass carbon electrode (GCE). The characterizations of CEC-Hb film were demonstrated by ultraviolet-visible (UV-vis) spectra, scanning electron microscopy (SEM), and electrochemical impedance spectroscopy (EIS). The electrochemical behaviors of Hb in CEC film have been investigated and a pair of well-defined and quasi-reversible cyclic voltammetric peaks for the protein heme Fe(III)/Fe(II) redox couples was observed at about -0.369V (vs. SCE). The CEC-Hb film exhibited a good electrocatalytic activity for the reduction of nitric oxide (NO). The amperometric response of the biosensor varied linearly with the NO concentration ranging from 1.1 x 10(-6) to 1.3 x 10(-4) mol L-1. Moreover, the studied biosensor exhibited high sensibility, good reproducibility, and long-term stability. Finally, this method has applied to monitoring the NO release from biologic samples. (C) 2009 Elsevier B.V. All rights reserved.
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