Journal
BIOSENSORS & BIOELECTRONICS
Volume 24, Issue 12, Pages 3455-3460Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2009.04.034
Keywords
Leaky surface acoustic wave (LSAW); Peptide nucleic acid (PNA); Biosensor; Human papilloma virus (HPV)
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Funding
- Major Project Chinese National Programs for High Technology Research and Development [2007AA02Z416]
- National Natural Science Foundation of China [30400107]
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A novel leaky surface acoustic wave (LSAW) bis-peptide nucleic acid (bis-PNA) biosensor with double two-port resonators has been constructed successfully for the quantitative detection of human papilloma virus (HPV). The bis-PNA probe can directly detect HPV genomic DNA without polymerase chain reaction (PCR) amplification, and it can bind to the target DNA sequences more effectively and specifically than a DNA probe. When the concentrations varied from 1 pg/L to 1000 mu g/L, with 100 mu g/L being the optimal, a typical linearity was found between the quantity of target and the phase shifts. The detection limit was 1.21 pg/L and the clinical specificity was 97.22% of that of real-time PCR. The bis-PNA probe was able to distinguish sequences that differ only in one base. Both the intraassay and interassay coefficients of variance (CVs) were <10%, and the biosensor can be regenerated for ten times without appreciable loss of activity. Therefore, this technical platform of LSAW biosensor can be applied to clinical samples for direct HPV detection. (C) 2009 Elsevier B.V. All rights reserved.
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