Journal
BIOSENSORS & BIOELECTRONICS
Volume 24, Issue 12, Pages 3538-3543Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2009.05.013
Keywords
Biosensor; Microalgae; Fluorescence; Simazine; Herbicides
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Funding
- Madrid Regional Government [S-0505/AMB/0374]
- ESF
- ERDF
- UCM [CCG07-UCM/AMB-2932]
- Isidro Parga Pondal Program (Xunta de Galicia)
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Three microalgal species (Dictyosphaerium chlorelloides (D.c.), Scenedesmus intermedius (S.i.) and Scenedesmus sp. (S.s.)) were encapsulated in silicate sol-gel matrices and the increase in the amount of chlorophyll fluorescence signal was used to quantify simazine. influence of several parameters on the preparation of the sensing layers has been evaluated: effect of pH on sol-gel gelation time; effect of algae density on sensor response; influence of glycerol (%) on the membrane stability. Long term stability was also tested and the fluorescence signal from biosensors remained stable for at least 3 weeks. D.c. biosensor presented the lowest detection limits for simazine (3.6 mu g L-1) and the broadest dynamic calibration range (19-860 mu g L-1) with IC50 125 +/- 14 mu g L-1. Biosensor was validated by HPLC with UV/DAD detection. The biosensor showed response to those herbicides that inhibit the photosynthesis at photosystem II (triazines: simazine, atrazine, propazine, terbuthylazine: urea based herbicides: linuron). However, no significant increases of fluorescence response was obtained for similar concentrations of 2,4-D (hormonal herbicide) or Cu(II). The combined use of two biosensors that use two different genotypes, sensitive and resistant to simazine, jointly allowed improving microalgae biosensor specificity. (C) 2009 Elsevier B.V. All rights reserved.
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