Journal
BIOSENSORS & BIOELECTRONICS
Volume 24, Issue 11, Pages 3383-3386Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2009.04.017
Keywords
16S rRNA; Electrochemical biochip; Esterase; Dendrimer; Detection of bacteria
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Funding
- Forschungskreis der Ernahrungsindustrie e. V.
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Novel enzyme-oligodeoxynucleotide conjugate was synthesized to improve sensitivity of Escherichia coli 16S rRNA detection on gold electrodes. Thermostable esterase 2 from Alicyclobacillus acidocaldarius was multiply conjugated to a polyamidoamine dendrimer functionalized by one universal detector oligodeoxynucleotide. Three components rRNA/DNA hybridization between capture oligodeoxynucleotide covalently immobilized on a gold electrode, 16S rRNA and the multivalent esterase-dendrimer cluster was used for detection of E. coli. The linear dependence of the electrochemical signals to analyte concentration revealed a detection limit of 50 colony forming units E. coli, which represents a tenfold signal enhancement if compared to the detection limit achieved with monovalent esterase-oligodeoxynucleotide conjugate. (C) 2009 Elsevier B.V. All rights reserved.
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