Molecularly imprinted nanopatterns for the recognition of biological warfare agent ricin

Molecularly imprinted polymer (MIP) for biological warfare agent (BWA) ricin was synthesized using silanes in order to avoid harsh environments during the synthesis of MIR The synthesized MIP was utilize for the recognition of ricin. The complete removal of ricin from polymer was confirmed by fluorescene spectrometer and SEM-EDAX SEM and EDAX studies confirmed the attachment of silane polymer on the surface of silica gel matrix. SEM image of Ricin-MIP exhibited nanopatterns and it was found to be entire different from the SEM image of non-imprinted polymer (NIP). BET surface area analysis revealed me surface area (227 m(2)/g) for Ricin-MIP than that of NIP (143 m(2)/g). In addition, surface area study all showed more pore volume (0.5010 cm(3)/g) for Ricin-MIP than that of NIP (0.2828 cm(3)/g) at 12 nm pore diameter confirming the presence of imprinted sites for ricin as the reported diameter of ricin is 12 The recognition and rebinding ability of the Ricin-MIP was tested in aqueous solution. Ricin-MIP reboue, more ricin when compared to the NIP. Chromatogram obtained with Ricin-MIP exhibited two peaks do, to imprinting, however, chromatogrant of NIP exhibited only one peak for free ricin. SDS-PAGE rest confirmed the second peak observed in chromatogram of Ricin-MIP as ricin peak. Ricin-MIP exhibited an imprinting efficiency of 1.76 and it also showed 10% interference from the structurally similar prote abrin. (C) 2009 Elsevier B.V. All rights reserver: