Journal
BIOSENSORS & BIOELECTRONICS
Volume 24, Issue 6, Pages 1592-1597Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2008.08.035
Keywords
Immunodevice; Antibodies; Dielectrophoresis; Microfluidic device; Microparticles; Atrazine; Wine matrix effect
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Funding
- Ministry of Science and Technology [TEC2004-0121-E, TEC2004-06854-C03-03/MIC, IST-2003-508774]
- Ministry of Education, Culture, Sports, Science and Technology of Japan [19650121]
- Grants-in-Aid for Scientific Research [19650121] Funding Source: KAKEN
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The detection of atrazine using a novel optical immunosensing technique based on negative dielectrophoresis (n-DEP) in microfluidic channels is described. Atrazine is a toxic triazine herbicide within the most frequently used. Polystyrene microparticles (6 mu m diameters) modified with bovine serum albumin conjugated with atrazine (atrazine-BSA) were manipulated and captured when subjected to intense n-DEP electric fields. Specifically, particles were trapped when AC voltages with amplitudes of 10 V-peak and frequencies over 1 MHz were applied to the electrodes. The immunological reaction occurring on the particles for detecting atrazine is based on an indirect competitive assay using a secondary anti-mouse immunogloburin G (IgC) antibody labeled with fluorescein isothiocyanate. The microfluidic device, with three-dimensional microelectrodes, was fabricated comprising two caged areas, allowing two simultaneous measurements inside the same microfluidic channel. The performance of this n-DEP immunosensing technique was evaluated using wine samples. The immunodevice showed a limit of detection for atrazine in buffer samples of 0.11 mu g L-1 and in pre-treated wine samples of 6.8 mu g L-1; these detection limits are lower than the maximum residue level (MRL) established by the European Community for residues of this herbicide in wine (50 mu g L-1). This methodology offers great promise for rapid, simple, cost effective, and on-site analysis of biological, foods and beverages, and environmental samples. (C) 2008 Elsevier B.V. All rights reserved.
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