4.8 Article

Rhodamine B isothiocyanate-modified Ag nanoaggregates on dielectric beads: A novel surface-enhanced Raman scattering and fluorescent imaging material

Journal

BIOSENSORS & BIOELECTRONICS
Volume 24, Issue 7, Pages 1864-1869

Publisher

ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2008.09.017

Keywords

Surface-enhanced Raman scattering; Fluorescence; Silica bead; Polystyrene bead; Rhodamine B isothiocyanate; Biotin-streptavidin interaction

Funding

  1. Korea Science and Engineering Foundation [R01-2006-000-10017-0, M1070300106707M0300-06711, RII-2007-012-02002-0]
  2. Soongsil University Research
  3. National Research Foundation of Korea [R01-2006-000-10017-0, 2007-0056334, 과06A1501] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)

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Rhodamine B isothiocyanate (RhBITC) is a prototype dye molecule that is widely used as a fluorescent tag in a variety of biological applications. We report in this work that once RhBITC is adsorbed onto Ag on silica or polystyrene beads, it exhibits not only a strong surface-enhanced Raman scattering (SERS) signal but also a measurable amount of fluorescence. The RhBITC-modified Ag-deposited silica or polystyrene beads disperse well in ethanol, and they are also readily coated in water with polyelectrolytes for their further derivatization with biological molecules of interest that can bind to target molecules. The application prospects of these materials are thus expected to be very high especially in the areas of biological sensing and recognition that rely heavily on optical and spectroscopic properties. For instance, on the basis of the nature ofthe SERS peaks of RhBrFC, those Ag-deposited silica or polystyrene beads were confirmed, after attaching biotin groups over RhBITC, to selectively recognize streptavidin molecules down to concentrations of 10(-13) M based on a signal-to-noise ratio of 3. The biotin-streptavidin interaction was also confirmed from the photoluminescence of RhBITC. (C) 2008 Elsevier B.V. All rights reserved.

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