Journal
BIOSENSORS & BIOELECTRONICS
Volume 24, Issue 8, Pages 2611-2617Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2009.01.016
Keywords
Poly(ethylene-co-vinyl alcohol); Molecularly imprinted polymers; Urinalysis; Potentiostat sensors
Categories
Funding
- National Science Council of the Republic of China, Taiwan [96-2220-E-390-001, 97-2220-E-006-011]
Ask authors/readers for more resources
Among many important biomarkers excreted in urine are albumin, uric acid, glucose, urea, creatine and creatinine. In the growing elderly population, these biomarkers may be useful correlates with kidney dysfunction, infection and related problems such as glomerular, proximal, and distal convoluted tubule functions, diabetes, hypertension and proteinuria. This study employed solvent evaporation processing of poly(ethylene-co-vinyl alcohol), (EVAL) to form molecularly imprinted polymers (MIPs) that recognize creatinine, urea, and lysozyme. The mole ratio of ethylene to vinyl alcohol affected the performance: 27 mol% ethylene gave the highest imprinting effectiveness for creatinine and urea, while 44 mol% gave the highest effectiveness for lysozyme. Electrochemical examination using a home made potentiostat and imprinted polymer electrode showed electrical signals responsive to the target molecules. Finally, an actual urine sample was tested using the electrode. The test results were compared with those of the commercial instrument ARCHITECT ci 8200 system to precisely determine the accuracy of the molecularly imprinted polymer electrode for urinalysis. (c) 2009 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available