Journal
BIOSENSORS & BIOELECTRONICS
Volume 23, Issue 12, Pages 1856-1861Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2008.03.005
Keywords
biosensor; Escherichia coli (E. coli JM109); bacteria detection; antibody immobilization; bacteria immobilization; interdigitated electrode array
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Real-time and specific detection of single bacterium remains a fundamental challenge and draws very much attention. Using test patterns composed of interdigitated Au-electrode arrays modified with antibody, the specific and quantitative detection of the electrical conductivity of a single Escherichia coli (E. coli, JM109) has been carried out in this work. The key is to ensure low background current of the antibody-modified test patterns before bacteria detection (< 0.7 pA in this case) and minimize the residual moisture or hydration after E. coli immobilization, such as via the use of 1-min bake at 50 degrees C prior to electrical measurement. This method holds great potential for future application in the real-time, specific, and quantitative bacterium detection down to a single bacterium cell. (c) 2008 Elsevier B.V. All rights reserved.
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