Journal
BIOSENSORS & BIOELECTRONICS
Volume 24, Issue 2, Pages 178-183Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2008.03.035
Keywords
protein A; immunoassay; surface-enhanced Raman scattering; molecular probe; Au nanoparticle
Categories
Funding
- Landmark Project of National Cheng Kung University [A0011, A0052]
- Technology Development Program for Academia of Ministry of Economic Affairs [96-EC-17-A-10-S1-013]
- National Science Council of the Republic of China [NSC 95-2221-E-006 -215-]
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A unique, sensitive, highly specific, and photobleaching-resistant immunoassay system utilizing gold nanoparticles and surface-enhanced Raman scattering (SERS) is described. This new system, featuring a capability of bifunctional analysis, is manufactured by chemisorption of antibody immunoglobulin G (IgG) on gold nanoparticles (AuNP), followed by coupling the Raman-active reporter molecule, 5,5 '-dithiobis(2-nitrobenzoic acid) (DTNB) to the surface of IgG-AuNP. The adsorbed DTNB molecules exhibit strong Raman signals via both electromagnetic and chemical enhancement. The narrow spectral widths and high photostability assure the system to be an excellent detection label. This SERS-based immunoassay is applied to the detection of protein A, which is a specific surface antigen of Staphylococcus aureus. A working curve is obtained by plotting the intensity of the SERS signal of symmetric NO2 stretching of DTNB at 1333 cm(-1) versus the concentration of the analyte (antigen). A dynamic range of two to three orders of magnitude and a detection limit of 1 pg/mL of protein A are achieved. (C) 2008 Elsevier B.V. All rights reserved.
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