Journal
BIOSENSORS & BIOELECTRONICS
Volume 24, Issue 1, Pages 162-166Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2008.04.010
Keywords
sensor; self-assembled monolayer; surface molecular imprinting; protein; potentiometer
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Surface molecular imprinting, as compared to molecular imprinted bulk polymers, has the advantages of higher re-occupation percentage of the reception sites, fast response, integration of sensing element and transducer. etc. In this study, a potentiometric protein sensor was developed based on the surface molecular imprinting technique. Using the self-assembled monolayers of alkanethiol with hydroxyl terminal groups as the matrix material, and target protein molecules as the template, the sensing layer was created on the surface of the gold-coated silicon chip-an electrochemical transducer. Potentiometric measurement demonstrated that the sensor could selectively detect myoglobin or hemoglobin molecules. either with or without the presence of other protein molecules in the same solution. (C) 2008 Elsevier B.V. All rights reserved.
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