Journal
BIOSENSORS & BIOELECTRONICS
Volume 24, Issue 2, Pages 279-283Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2008.03.046
Keywords
biosensor; field effect transistor; carbon nanotubes; bacteria; Salmonella
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Funding
- Spanish Ministry of Education and Science
- MEC [NAN2004-09306-C05-05, CTQ2007-67570]
- Spanish Ministry of Science and Technology
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In this paper we report a fast, sensitive and label-free biosensor for the selective determination of Salmonella Infantis. It is based on a field effect transistor (FET) in which a network of single-walled carbon nantotubes (SWCNTs) acts as the conductor channel. Anti-Salmonella antibodies were adsorbed onto the SWCNTs and subsequently the SWCNTs were protected with Tween 20 to prevent the non-specific binding of other bacteria or proteins. Our FET devices were exposed to increasing concentrations of S. Infantis and were able to detect at least 100 cfu/mL in 1 h. To evaluate the selectivity of our FET devices, Streptococcus pyogenes and Shigella sonnei were tested as potential competing bacteria for Salmonella. At a concentration of 500 cfu/mL, neither Streptococcus nor Shigella interfered with the detection of Salmonella. Therefore, these devices could be used as useful label-free platforms to detect S. Infantis and, by using the suitable antibody, other bacteria or viruses. (C) 2008 Elsevier B.V. All rights reserved.
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